Abstract The use of biopolymers and platelet-rich fibrin (PRF) is currently investigated as an excellent application biomaterial in the tissue engineering field. Natural biomaterials with application potential in tissue engineering and regenerative medicine are due to their characteristics in biocompatibility, biodegradability, and mechanical characteristic. Though, the basic knowledge of three‐dimensional fibrin scaffold and chitosan/alginate processing technology combined with an understanding of the physical-chemical properties of this natural biomaterial is necessary for proper application in regenerative medicine and tissue engineering. This mini review summarizes the information on the composition of chitosan/alginate and PRF in medical area and discusses recent developments with a special focus on its use for the application of drug delivery. The most important advances and stimulating applications are studies in regenerative medicine and tissue engineering, for wound healing to treat burns, tumor treatment, nanoparticle carriers, and drug delivery systems.

Abstract The use of Wharton's Jelly (WJ) as a biomaterial is currently undergoing an appearance in the regenerative medicine field. The biomaterials applications focus on the aspects of cellular growth or delivery of proteins capable of stimulating cellular response. However, the basic knowledge about Wharton jelly and decellularization processing technology combined with understanding the physical-chemical properties of this biomaterial is necessary for proper application in regenerative medicine. This mini-review article summarizes information on the composition of WJ, application of drug delivery, in medicine and discusses recent developments with a special focus on its use for regenerative medicine. The most successful and stimulating applications are studies in regenerative medicine and tissue engineering, for wound healing to treat burns, tumor treatment, nanoparticle carriers, and drug delivery systems.

A fim de realizar a quantificação de coliformes totais e fecais no decorrer das etapas de tratamento da água captada pela Companhia de Saneamento do Paraná (SANEPAR) Curitiba-PR, foram utilizadas três técnicas de análise microbiológica: (1) a técnica oficial preconizada pela Vigilância Sanitária, (2) Kit "A", (3) Kit "B". As metodologias foram validadas através de controles positivos e negativos. Os métodos utilizados apresentaram resultados extremamente homogêneos nas amostras pré-tratadas e também quando as amostras haviam passado pelos processos de purificação, porém, algumas discrepâncias entre os métodos avaliados foram detectadas em etapas intermediárias do tratamento da água. A utilização de Kits no controle microbiológico da água é muito prática porque elimina as etapas de preparação e esterilização dos meios de cultura em laboratório, porém deve-se analisar cuidadosamente o custo-benefício, bem como a sensibilidade e especificidade dos produtos disponíveis no mercado. O Kit "B" emprega um método enzimático, ao invés do método tradicional e Kit "A", que utilizam ambos: a turvação e formação de gás para a detecção o crescimento bacteriano. O kit " B" apresentou resultados similares aos outros dois métodos, mas com as vantagens de apresentar resultados extremamente claros e levar a metade do tempo para a obtenção dos resultados.

In order to carry out the quantification of total and fecal coliforms in elapsing of the stages of water treatment by SANEPAR, Paraná State, three different techniques of microbiological analysis had been used: (1) the official technique praised by the Sanitary Monitoring; (2) Kit "A" and (3) Kit "B". The three methodologies of the water microbiological analysis had been validated through positive and negative controls. The used methods had presented extremely homogeneous results when the samples were contaminated and also when the samples had passed through the purification processes, however, some discrepancies among the evaluated methods had been detected in intermediate stages of the water treatment. The use of commercial Kits in water microbiological control is very practical because it eliminates the preparation and sterilization stages of the culture media in laboratory, however, it is necessary to analise carefully the cost-bennefit, sensibility and specifity of disposable products in market. The Kit "B" utilizes an enzymatic method, instead the tradicional technique and Kit "A" use both: the dim and gas formation to detect bacterial growth. The Kit "B" had showed similar results to the other two techniques, but with the advantages of presenting extreme clear results and take a half of time in the results obtention.